Nena

Nena is a minor antagonist appearing in Part III: Stardust Crusaders.

Personality
Nena is introduced as a tsundere-like girl, having a crush on Hol Horse to the point of marriage. Not satisfied with her actual appearance, she hides her true form even from her allies and uses the good looks of her last victim to trick her opponents into thinking she's just a innocent woman. Enya Gail notes that her especialty is to slowly kill her victims.

History
Nena is an ugly woman from a aristocratic family hired by Dio Brando to kill the Joestar group. Nena used her stand Empress to kill a beautiful young woman and wear her skin to hide her true form. She eventually falls in love with Hol Horse to the point of wanting to marry him. The two become ally and lovers, but Hol Horse actually was just using her love because, like many women around the world, she would be willing to do anything for him.

Stardust Crusaders
Nena is initially portrayed as an ally and lover of Hol Horse, and enemy of the protagonists, but not a true enemy herself. She shows interest in marrying him, but he denies her marriage offer claiming he is a traveler destined to die on the street and couldn't stay with her even though they loved each other. He later reveals to J. Geil that he is with her just because she could be useful in the future, sacrificing her life for him.

After Hol Horse is defeated she eventually comes to his rescue, holding Jean Pierre Polnareff while Hol Horse escapes from Jotaro's team. She then accompanies the group on a bus they take to Varanashi, and it is then that Jean Pierre Polnareff becomes attracted to her while she hides her actual identity as a Stand User, and attacks Joseph Joestar with her stand.

Nena then uses Polnareff´s interest in her to distract him while her stand fights Joseph. After Joseph is able to deal the finishing blow to The Empress, Nena takes the damage and dies, revealing her real form.

Stand
Nena's stand, Empress, represents the Tarot Card The Empress, which symbolizes fertility. The Empress card often puts emphasis to motherly qualities, like creation of life, and it is thought to signify physical attraction and luxury. Nena's luxury can be seen through her crush on Hol Horse and later attraction towards Polnareff (even though she was only faking the later). Her stand, Empress, refers to her fertility by continuous calling Joseph its daddy while trying to kill him, while Nena would be its mother. As a way to answer the stand, who continues to demand "his daddy" to feed him, Joseph tells him he shouldn't rely on his parents forever, minutes before destroying the stand completelly.

Trivia

 * She and Hol Horse were shown to be lovers, just like the tarot cards they represent (The Empress and The Emperor), though it is unknown if Hol Horse knew her true appearance.
 * Nena appears in the OVA, but her role as a antagonist is excluded. As such, she only appears to allow Hol Horse to escape.