Valkyrie (ヴァルキリー,Varukirī) is a 4-year-old stock horse with Australian roots and the horse that Gyro Zeppeli rides in the Steel Ball Run race. Gyro specifically chose the horse for its stamina and durability. As noted by Diego Brando, Valkyrie has a quirk where it sways suddenly to its left for every eight breaths it takes.
After the race is over, Johnny brings the horse back to Naples.
Slow Dancer (スロー・ダンサー,Surō Dansā) is an 11-year-old appaloosa and the horse that Johnny Joestar rides. Initially, it proves difficult to tame, but by the start of the race, it manages to warm up to Johnny and hoists Johnny onto the saddle by hoisting him and causing him to roll along its neck. Though Slow Dancer is old, it does not lack in stamina, and Gyro states that choosing the old horse is better due to the horse's experience, as opposed to the younger horses that hold much power but charge into danger without an afterthought.
Hey! Ya! (ヘイ！ヤア！,Hei! Yaa!) is a 4-year-old quarter horse that Pocoloco rides. Despite the lack of skill demonstrated by both the rider and the horse, Hey! Ya! manages to survive and propel Pocoloco to second place in the finish line after Diego Brando (this changes to first after Diego's "disappearance").
Roxanne (ロクサーヌ,Rokusānu) is 5-year-old Arab ridden by Indian racer Zenyatta Mondatta. His rider is also named after an album by Police. Zenyatta and Mondatta were Oingo and Boingo's names in Viz's American translation.
Black Rose (ブラックローズ,Burakku Rōzu) is a big black horse that is a bit sensitive. Diego Brando was leading the horse while Nicholas Joestar got on top of it. Nicholas intended to ride the horse in his next tournament.
Nicholas was capable of controlling the horse perfectly despite its size. However, he falls off the horse after some time and ends up dying. Diego says that he saw a white mouse scuttling near Black Rose's legs, which startled it and caused Nicholas to fall.