Josuke and Okuyasu come across a peculiar person named Mikitaka Hazekura who claims to be an alien. Believing his claims to be a joke, Josuke initially suspects him to be a Stand user when he exhibits some strange abilities. However, upon being distressed by the sound of sirens, Mikitaka suddenly transforms his body into a pair of sneakers and flees with Josuke, who realizes he is not a Stand user as he cannot see Crazy Diamond. Looking to take advantage of Mikitaka's transformation ability, Josuke has Mikitaka transform into some dice and challenges Rohan to a game of Cee-loW in the hopes of winning some money off of him. As the match begins, Josuke grows worried when Mikitaka seems to get suspiciously ideal rolls.
Becoming suspicious of Josuke's winning streak, Rohan stabs his own finger, staking two million yen against Josuke's own finger to determine how he is cheating while also calling upon Tamami Kobayashi as an official to prevent Josuke from playing normally. Just as Mikitaka becomes unnerved by sirens and starts to lose his transformation, Rohan is distracted upon discovering he had accidentally set his house on fire, allowing Josuke to escape without being discovered. The next day, while riding a bus through a tunnel with Josuke, Rohan spots a window to a room revealing a man cutting off a woman's hand, believing it to be Kira. Returning to inspect the tunnel, Rohan discovers the room to be an illusional trap, suddenly finding himself attacked by a nutrient-sucking Stand named Highway Star. When Josuke comes to the tunnel, Highway Star tries to use Rohan to lure him into his room. As Josuke ignores Rohan's warnings and enters the room anyway, Rohan sends him flying out of the tunnel, urging him to flee from Highway Star and find his user.
Believing there is a connection between Highway Star's user and the Futatsumori Tunnel he resided in, Josuke gets in contact with Koichi, who suspects the user to be someone hospitalized from a traffic accident stealing nutrients to heal himself. While Josuke makes his way to the hospital while keeping ahead of Highway Star, Koichi manages to find out the identity of its user, Yuya Fungami. Despite being caught by Highway Star upon reaching Yuya's room, Josuke manages to recover his nutrients with an IV drip, healing Yuya's injuries so he can pummel him without guilt. Meanwhile, at the Kawajiri household, Shinobu finds a British Blue cat hanging around the basement.
Upon discovering the cat to have a hole in its throat, Shinobu sends Kira down to the basement, where he discovers the cat had been killed by glass shards during its struggle with Shinobu. After being buried in the garden, the cat mysteriously comes back to life in the form of a strange plant known as Stray Cat, possessing the ability to control air. Discovering Stray Cat to be the cat from earlier and realizing it has a strong grudge against Shinobu, Kira ends up shielding her from its attacks, eventually stopping it by appealing to its cat-like nature. The next day, Hayato discovers Stray Cat while investigating the attic, coming under attack after he exposes him to sunlight. Managing to use Stray Cat's air attacks to put it back to sleep, Hayato barely manages to avoid being discovered by Kira, who he realizes isn't his real father.
Yoshihiro, recognizing Kira in his new appearance, notices he is being followed by Hayato. Later, on July 15th, Josuke and Okuyasu come across Mikitaka, who spots a Stand user named Toyohiro Kanedaichi living on an electrical pylon. When Josuke spots Yoshihiro with him, he steps inside the pylon and falls under the spell of Toyohiro's Stand, Super Fly, becoming trapped inside until someone else takes his place. Josuke and Okuyasu attempt to destroy the pylon, only to discover it dishes out any damage it takes. Wanting to help, Mikitaka confronts Toyohiro, helping Josuke escape but becoming trapped himself. Meanwhile, as something troubling happens in the Higashikata household, Rohan, who had noticed Hayato's behavior, is approached by a strange man.
Rohan is greeted by Masazo Kinoto, an architect sent to evaluate the damages done to his house, using his Heaven's Door to find nothing suspicious besides an aversion to people looking at his back. Meanwhile, Josuke climbs up the pylon to rescue Mikitaka and confront Kanedaichi, who uses calculative cuts on the pylon to attack Josuke from all angles. However, Josuke manages to use Crazy Diamond's healing ability to send the bolts of energy back to their origin and defeat Kanedaichi. Resigning himself to stay inside the pylon, Kanedachi reveals that he heard Yoshihiro say that another Stand user has allegedly eliminated Koichi. Enlisting Yuya's help in tracking Koichi's scent in exchange for healing his injuries, Josuke encounters the Stand user, Terunosuke Miyamoto, who unbeknownst to him had used his Stand Enigma to turn Koichi and Tomoko into pieces of paper after witnessing their ticks when they are afraid.
Overcome by his curiosity, Rohan lays a trap for Kinoto so he can see his back. The moment he does, however, a Stand possessing its own will named Cheap Trick bursts from Kinoto's back and attaches himself to Rohan's back instead, demanding that he burn the pictures he had taken of Kira suspects. Meanwhile, Miyamoto reveals that he has trapped Koichi in a piece of paper, managing to prompt Josuke into biting his lip and falling under Enigma's power himself, becoming trapped as well as a result. Despite not wanting to get involved, Yuya becomes moved by Josuke's resolve and goes after Miyamoto, encountering several paper-hidden traps that eventually culminates in a shredder that threatens to shred both Josuke and Koichi. Despite being overcome by fear and falling under Enigma's power, Yuya manages to take advantage of his now paper-like state to reach into the shredder and free Josuke and Koichi, who quickly deliver their vengeance on Miyamoto who winds up in the form of a book.
Unable to get Koichi to take him seriously without exposing his back and passing Cheap Trick onto him, Rohan attempts to make his way to Morioh Grand Hotel without letting anyone see his back. Cheap Trick attempts to attract several cats and dogs to attack Rohan and witness his back, but they are shooed off by Koichi, who eventually comes to believe Rohan's story. Koichi attempts to forcibly remove Cheap Trick using Echoes ACT3, only to discover that doing so will damage Rohan as well. Despite this, Rohan succeeds in his ultimate goal to lure Cheap Trick into Reimi's Ghost Alley and trick him into turning around, getting dragged into HellW by the alley's hands as a result. Meanwhile, Hayato witnesses Kira killing a couple with his ability and catches it on film, something that Kira is quick to notice. Kira confronts Hayato and prepares to kill him, only for Hayato to reveal he has set up further cameras and videotapes that would threaten to expose his identity.
Kira inadvertently kills Hayato before learning from Yoshihiro that Rohan and the others now have Kosaku Kawajiri as one of their suspects. As Kira becomes anxious over what he should do, he is suddenly impaled by Yoshihiro's Arrow, giving Killer Queen a new ability. The next morning, Hayato appears alive and well, as if nothing had happened to him the previous night, noticing Kira has strange confidence about his position. Later that morning, Hayato comes across Rohan, who uses Heaven's Door to read his memories. After reading several pages predicting occurrences moments before they happen, Rohan comes across a page revealing Kira's identity. It is at this moment, however, that Kira activates the third bomb that he had implanted in Hayato, Bites the Dust, which is set to kill anyone who learns about Kira's identity through Hayato. As the explosion goes off inside of Rohan's body, Hayato suddenly finds himself sent back in time to an hour earlier due to Bites the Dust's effects.
Hayato tries to avoid meeting Rohan, only to discover the events that occurred last time will still happen even without him being there, resulting in Rohan's death by Bites the Dust's explosion. When Josuke's group arrive at the scene shortly afterwards, Hayato attempts to kill himself to avoid being found out. However, he is stopped by Killer Queen, who sets off Bites the Dust in Josuke and the others and once again returns Hayato to the start of the morning. Realizing that he can only stop Kira by either killing him or forcing him to undo Bites the Dust, Hayato hides Stray Cat in his backpack, launching a surprise attack on Kira when he follows him later in the morning. However, because of a slight alteration of events due to Hayato's actions, Kira manages to protect himself from Stray Cat's attack with a watch he had put in his jacket.
As Kira brags about his victory to Hayato, he unknowingly reveals his own identity to Josuke, who Hayato had secretly called ahead of time. Needing Killer Queen to protect himself, Kira is forced to undo Bites the Dust, preventing Rohan from being killed by its effects. Engaging in battle against Josuke and Okuyasu, Kira manages to retrieve Stray Cat and use its air shots to create invisible remote bombs, dealing heavy damage to Okuyasu. As Josuke tries to reach Okuyasu in order to heal him, Hayato deduces that Okuyasu's body might have been turned into a bomb, leaving Josuke with the tough decision of whether to sacrifice himself to save Okuyasu. However, Hayato, deducing that Kira can only set off one bomb at a time, sets off the bomb on himself, allowing Josuke to heal both him and Okuyasu. As Kira launches another air bomb, Josuke, refusing to believe Okuyasu might already be dead, manages to use Crazy Diamond on debris in his wounds to dodge the explosion.
Despite Josuke and Hayato hiding inside the house, Kira manages to figure out Josuke's location and send an air bomb after him. Although Josuke gets hurt in the explosion, he manages to use his hardened blood on a vase fragment, turning it into a homing bullet that manages to hit Kira. Noticing Kira carrying a cell phone with him, Josuke deduces that Yoshihiro had been hiding in Hayato's pocket, informing his son of their location, and tricks Kira into killing him instead. Josuke then draws Kira into a close quarters fight, but Stray Cat manages to protect Kira from Crazy Diamond's attacks. Just as Josuke is about to be hit by another air bomb, he is saved by a very much alive Okuyasu, who retrieves Stray Cat from Kira, driving him further into a corner as Jotaro and the others arrive on the scene.
Surrounded by ambulances and fire engines, Kira attempts to use an approaching nurse to activate Bites the Dust again. While initially believing himself to have succeeded, Kira finds himself in Ghost Alley. There, Reimi reveals to Kira that he had, in fact, died after he was stopped by Koichi and Jotaro and run over by an ambulance. Thanks to the combined efforts of Reimi and Arnold, Kira is forced to turn around and is dragged into the underworld by the alley's hands. No longer bound to her attachments, Reimi and Arnold bid farewell to everyone before moving on to the next life. The next day, Jotaro and Josuke return home as the summer of 1999 draws to a close for the residents of Morioh Town.
In 2001: 2 years after the battle against Yoshikage Kira, Koichi Hirose travels to Italy by Jotaro's favor. He departs Naples Airport to find a boy by the name of Haruno Shiobana. Koichi comes across a peculiar young man who tricks him and drives off with his luggage. Koichi overhears two airport security guards reveal the young man's name as Giorno Giovanna, and infers that Giorno might be Haruno Shiobana. Meanwhile, Giorno meets with the gangster Leaky-Eye Luca, who attacks Giorno and is put into a coma after his attack is reflected back. Giorno reveals his Stand, Gold Experience, and creates a tree to escape from Koichi who caught up to him. Koichi calls Jotaro by telephone, revealing Giorno is the son of DIO. Escaping by train, Giorno encounters Bruno Bucciarati who questions him about Luca's death. Giorno lies to him and Bucciarati goes away. After, Giorno opens his hand only to find an eye enclosed, and Bucciarati reappears behind him and confirms he's lying by licking his face.
In a flashback, it is revealed that Giorno, who was once abused by his step-father and bullied by other kids, began being treated with respect after saving an injured gangster, giving him a reason to live. Back in the present, Giorno uses Gold Experience against Bucciarati, which causes his senses to go berserk upon being hit. Bucciarati brings out his own Stand, Sticky Fingers, allowing him to create a dimension distorting zipper to try and escape inside another person, but Giorno tracks him down by turning one of Bucciarati's teeth into a fly. Despite beating Bucciarati in a duel, Giorno decides against finishing him off, and instead asks to join his organization so he can take down his boss and take over the city.
Telling Bucciarati about his ambition, Giorno convinces Bucciarati to let him join the organization Passione and Bucciarati leads him to a lieutenant of the gang Polpo, who is currently sitting in prison. After entering the prison, Giorno asks to join the gang and is assigned a test in order to see if he is trustworthy- which is to guard a lighter's flame for 24 hours. Giorno accepts and leaves the prison, and after a series of close calls, manages to get into his dorm, only for Koichi to intrude in order to find his stolen passport. Giorno again narrowly escapes with the lighter however the lighter is extinguished accidentally by a janitor cleaning the area. Distraught, Giorno believes he has blown his chance. When against expectations the janitor helps Giorno reignite the lighter, Black Sabbath appears and impales the janitor's soul with an Arrow, killing him. Black Sabbath then detects Giorno and attacks the latter.
Black Sabbath tries to attack Giorno reveals itself to be more powerful than Gold Experience as well as swifter. After a lucky sun ray stuns the Stand, allowing Giorno to escape its grip, the young boy understands that Black Sabbath is weakened by sunlight but powerful in the shades. When Koichi comes, the two teens ally to lure Black Sabbath into the light and annihilate it. Afterward, Giorno convinces Koichi of his righteousness and also makes him keep his secret goal. Koichi thus reassures Jotaro about Giorno and departs from the latter's life. The next day, Giorno is welcomed by Polpo into the gang and officially assigned to Bucciarati. But as revenge for the janitor, he secretly turns one of the lieutenant's guns into a banana. Long after Giorno steps out of prison, Polpo finally eats the banana and kills himself with a shot to the head. Meanwhile, Giorno is on his way to meet Bucciarati's Gang.
While Bucciarati explains how Passione functions as a gang, the news of Polpo's death spreads throughout the mob. Two gangsters, Sale and Mario Zucchero, take an interest in Polpo's hidden treasure that Bucciarati supposedly knows of. Giorno formally meets his new teammates and manages to make a good impression despite them antagonizing Giorno. Learning about Polpo's death, Bucciarati immediately tells the gang to set sails. On their way to Capri in order to retrieve the treasure, an unseen enemy Stand ambushes them, making Narancia, Mista, and Fugo disappear without a trace. As Abbacchio is reluctant to show his Stand to the newbie, Giorno makes the gamble that their opponent's way of attacking isn't fatal, and purposefully exposes himself. He is stabbed by the Stand and disappears. Thus Abbacchio is free to summon his Moody Blues.
Leone Abbacchio's past is revealed. Once a policeman corrupted by his surrounding, his moral downfall culminated in the murder of his partner in the police by the same criminal who had been bribing him. Dishonored, Abbacchio became desperate until he met Bucciarati. In the present, Bucciarati and Abbacchio begin to investigate the enemy's power, using Moody Blues' power to reenact any event from the past to discover how the enemy works. Although they discover that the enemy can deflate people and is probably hiding in the pipes, they cannot find him. However, Abbacchio sacrifices himself and uses blood to make Bucciarati understand that there is a second deflated boat covering the first one. Bucciarati thus sinks his yacht on purpose, forcing Zucchero to reveal himself and easily defeats him.
After Mario Zucchero is defeated, Bucciarati's team learns that he's told his yet unseen partner that they were headed to Capri to retrieve the treasure. As Bucciarati took a long curve to avoid being seen directly heading toward Capri, the group expects to fight another enemy on the island, but Giorno proposes to head there first and seek out their foe. Near the marina, Giorno calls for the enemy through a civilian while Mista lies in wait, ready to fight. The enemy Sale falls for the bait, and despite some mishaps, Mista manages to shoot Sale in the leg thanks to his Sex Pistols's power to redirect bullets. Mista pursues the enemy alone up to a starting truck, leaving Giorno behind.
Guido Mista's past is revealed: Mista was once a simple teenager enjoying life until he met his destiny and stopped a gang rape, revealing his outstanding gunfighting skill. In the present, Mista shoots Sale who was hidden atop the truck. Yet, Sale's Stand Kraft Work can immobilize objects with respects to a given reference point. Thus Mista is at a tremendous disadvantage as bullets cannot penetrate Sale's skin while he can even fire the bullets back at Mista. After a back and forth, Mista is left with one bullet and wastes it. However, he only tricked Sale into dropping his guard and has Sex Pistols hijack Sale's own bullet, which hits Sale right in the first bullet still locked inside Sale's skull. The bullet is pushed inside the brain and Sale is defeated, but alive.
Having succeeded in coming to Capri safely, Team Bucciarati meets with the capo Pericolo. Polpo's treasure which was hidden inside a restroom's walls is examined and deemed great enough, thus Bucciarati is officially made into a capo of the gang. However, Pericolo then presents Trish Una, the daughter of the Boss. Born from a long past tryst, Trish is now the target of a rogue team of Stand User assassins who hope to get their hands on her to get to the Boss, and Bucciarati's team must now bodyguard her. Later, Narancia is sent out of the hideout to buy groceries but is easily found by Formaggio, one of the said assassins. Formaggio's Little Feet claws at Narancia who unleashes his Aerosmith to fire at the car. Formaggio then uses his power to shrink himself and others to hide and wait for Narancia to shrink, discovering at the same time that Bucciarati's team is bodyguarding the Boss' daughter.
The fight between Narancia and Formaggio continues. Having shrunken himself and hiding inside Narancia's pocket, Formaggio patiently waits for his foe to shrink. However, Narancia understands what's happening to him and, to stop Narancia from calling for help, Formaggio must cut the wire of the phone Narancia uses. The boy realizes that Formaggio is nearby and uses an unknown ability to track Formaggio. Running away, Formaggio is relentlessly tracked into a drain. A flashback then explains how the Hitman Team, dissatisfied with their pay, had two of their members gruesomely executed for daring to pry into The Boss' past. In the present, Formaggio now understands that Aerosmith is tracking him because of the CO2 he releases. Using a swarm of rats, Formaggio tries to hide but Narancia manages to identify him and shoot. Although he is hit, Formaggio grows back to his original size while Narancia and Aerosmith have become so tiny that they are too weak at the moment.
Narancia has now shrunk to the point where he cannot do significant damage to Formaggio. Failing to hide, the assassin stomps him under his foot and resumes his interrogation, but Narancia keeps silent. Narancia's sad story is also revealed: once a street urchin and loyal to his little band of thieves, Narancia was one day framed for violent aggression by his comrade and was thrown into reform school for a year, only to be shunned when he got out. Ill and alone, Narancia escaped his squalor thanks to Fugo and Bucciarati, to whom he is now loyal to. In the now, an exasperated Formaggio throws Narancia inside a bottle with a spider in it and manages to learn where Trish is kept. However, Narancia reverses the situation by making his own car explode thanks to Aerosmith's tracer bullets, grows back to normal size, and then devastates the entire street to prevent the assassin from fleeing. Finishing Formaggio off, Narancia returns to the hideout where Bucciarati receives The Boss's second orders. He then sends Fugo, Abbacchio, and Giorno to Pompeii to retrieve a key.
Giorno, Fugo, and Abbacchio arrive at the famous ruins of Pompeii. As the trio is walking toward the dog mosaic, Fugo sees a mirror and a new enemy in the reflection. It is Illuso, one of the assassins from La Squadra Esecuzioni. As Illuso approaches, Fugo warns his teammates who cannot see the enemy and Fugo is suddenly snatched into the mirror world, confronting Illuso and his Stand, Man in the Mirror. Fugo then tries to summon his Stand Purple Haze but the Stand is kept out of the mirror, interrupting Giorno and Abbacchio's search for Fugo. A flashback exposes Fugo's past. As a well-born child with high intellect, Fugo was so pressured into academic excellence that it took a toll on his sanity. Entering a university at age 13, he finally snapped and beat up a professor to defend himself but was expelled then disowned, eventually meeting Bucciarati and being swayed to his side. Fugo tries his best to fight Man in the Mirror indirectly but Illuso easily bests him while Purple Haze's actions cause one of the capsules on his fist to crack, revealing its ability to release a deadly flesh-eating virus. Abbacchio decides to prioritize the objective but Giorno insists on helping Fugo, and Abbacchio leaves Fugo to his fate. Meanwhile, Illuso approaches Fugo for a coup de grâce.
The battle against Illuso and Man in the Mirror continues. Abbacchio reaches the key but Illuso has detected and followed him, neglecting to kill Fugo. Abbacchio sees Illuso inside of a mirror as well and understands the situation. Tricking Illuso into forcing a disguised Moody Blues to enter the mirror world and gaining the upper hand, Illuso nonetheless uses a piece of glass to trap both Stand and User half inside of the mirror, disabling both. Desperate, Abbacchio cuts off his hand to permit Moody Blues on the other side to bring back the key to Giorno's position and Illuso attacks Giorno. However, Giorno has infected himself with Purple Haze's virus, exposing Illuso who must flee to the real world. Nonetheless, Giorno has also turned a brick into a snake, allowing Fugo to guess Illuso's position thanks to the snake's heat sense. Purple Haze then dissolves Illuso. For his part, Giorno manages to save himself by using the snake's blood as an antivirus, greatly impressing his teammates. The team has acquired the key.
Discovering new instructions from the Boss engraved on the key, the group now heads to Naples' train station. Near the Florence-bound Express, Bucciarati discovers a turtle whose shell fits with the key, grabs it and rushes inside the train to his team, evading two new enemies that have been following him: Prosciutto and his junior Pesci. Perplexed by Bucciarati's sudden disappearance, the pair decides to stay on the train. Unbeknownst to the assassins, the small turtle acts as a stealthy shelter for Team Bucciarati thanks to its Stand ability and hides in a corner of the driver's cabin. To draw them out, Prosciutto summons his Stand The Grateful Dead and accelerates the aging of the train's passengers, sweeping the wagons while Pesci stands guard near the driver's cabin. Affected by the aging, the group discovers that ice can slow it down then sends out the relatively unaffected Mista out to fight the enemy. However, when Mista tries to activate the air conditioner, he is ambushed and is hooked by Pesci's fishing rod Stand, Beach Boy.
The battle between Mista and Pesci begins. Having hooked Mista by the hand and Beach Boy's line being unbreakable as well as able to reflect energy into the catch, Pesci seems to have the advantage and tries to reel Mista in, making the hook draw closer to the gunman's heart. Nonetheless, Mista turns the table on his foe by firing Sex Pistols and destroying his ice, making Pesci panic and unsummon Beach Boy. Mista confronts Pesci and almost shoots him but Prosciutto, disguised as an old man, takes Mista's hand and disables him. Lecturing Pesci again on his team's fighting spirit, Prosciutto shoots Mista in the head and goes back to the cabin. Prosciutto and Pesci eventually find the turtle but are attacked by Bucciarati, as Mista has survived thanks to Sex Pistols and informed his leader of the situation. However, Bucciarati's fight exhausts him and the aging effect takes hold, forcing the capo to throw himself out of the express train with Prosciutto and hopefully take him out.
The battle against Prosciutto and Pesci continues. Bucciarati has thrown himself out of the train with Prosciutto in his arms in the hopes of killing the assassin. Pesci intervenes to grab Prosciutto but Bucciarati returns Beach Boy's reflecting power against his enemy, zipping out Prosciutto's hand. Prosciutto seemingly falls to his death while Bucciarati is hooked. Pesci realizes the situation and despairs, but when he sees that Prosciutto has put himself inside the machinery of the train and maintained The Grateful Dead despite his wound, he is inspired by the example and changes into a cold-blooded killer. Pesci tries to kill Bucciarati who is forced to enter the train again and separate himself into many pieces to evade Beach Boy. However, he's cut his heartbeat and respiration to do so. Bucciarati almost dies but Pesci finally loses patience and drop his guard to stop the train. The two confront each other one last time and Bucciarati wins, pummelling Pesci into a river. The two assassins are dead. However, it seems that Trish is manifesting for the first time her Stand power and Melone the assassin is on the team's trail.
In the express train, Melone uses Bucciarati's blood sample and a woman he's deemed really incompatible with the former to create a Stand homunculus thanks to his Baby Face, teaching him how to kill Bucciarati and capture Trish before siccing him on the team. Meanwhile, Team Bucciarati is forced to stay on a parking lot and tries to steal a car. Giorno, carrying Coco Jumbo containing Trish and Bucciarati is temporarily isolated from the team. It is at this moment that Baby Face sneaks inside the turtle and turns both Trish and Bucciarati into cubes, then takes away Giorno's throat, a foot, and an eye. Crippled and suffocating, Giorno is at first utterly overwhelmed by Baby Face but then realizes from witnessing its cube power that he can shape objects into individual organs and heal. Managing to retake the turtle, Giorno outsmarts Baby Face and fakes having his hand cut off, turning the decoy hand into a piranha devouring its way out of Baby Face.
Now having the advantage against Baby Face, Giorno then makes short work of the homunculus despite its physical growth. With his victory, Bucciarati and Trish also return to normal. The assassin Ghiaccio arrives at the parking lot some time after, only seeing a bunch of stolen car. Although Melone assures on the phone that he can track the group again, he is suddenly killed by a venomous snake sent by Giorno. The assassins have seemingly lost track of their target but Risotto Nero their leader has managed to find another trail. Meanwhile, the group discovers by rewinding Moody Blues that Pericolo has set up further instructions for them: they must retrieve a disc hidden near Venice's train station to read how to deliver Trish. Moreover, Pericolo has shot himself, and The Boss is now awaiting Trish's arrival. Giorno and Mista are sent together retrieve the disc, but the duo is then confronted by Ghiaccio, who thanks to computer analysis on Pericolo's computer has managed to discover their destination. Ghiaccio's Stand White Album is a deadly Stand of cryogenic power which can kill through simple exposure and makes an invincible ice suit around Ghiaccio. Unable to shake him off, Giorno decides to drive his car into the sea.
The battle against Ghiaccio continues. Having driven into the sea, Giorno hopes to slow down White Album's freezing ability with salt water and tells Mista to get away while he stalls Ghiaccio. When Ghiaccio freezes the sea to demonstrate his power, Mista, and Giorno cooperate to create a grass sled from frozen car parts, allowing Mista to approach the shore. Then, turning the grass back into car parts, they manage to drive a screw into Ghiaccio's helmet and stun him. Surprisingly, Mista decides to fight Ghiaccio, discovers an air hole on White Album's neck area and shoots into it, but Ghiaccio activates his technique "White Album Gently Weeps" to create a barrier of frozen air, rebounding the bullet back at Mista. Now on solid ground, Mista confronts Ghiaccio again. Giorno sprays his blood in the air, exposing the weaknesses in the frozen air. Mista is also reminded of how Bucciarati helped him out of prison and enlisted him in the gang. Ghiaccio then simply shuts his airhole, using the frozen air as oxygen reserve. Nonetheless, Mista manages to impale Ghiaccio on a broken lamppost and tries to shoot him further. A bloody shootout ensues but Ghiaccio freezes his own blood into pillars, taking the advantage. At last, Giorno intervenes to save Mista and deals the finishing blow to Ghiaccio. Mista and Giorno have won the battle and awakened a great respect for one another.
Team Bucciarati have acquired the Boss' last orders: they must now head to the San Giorgio Maggiore church South of Venice, and one of them must accompany Trish to the top of the belfry, without weapons or communication tools while the rest waits inside the boat. It is a unique opportunity to approach the Boss so Giorno gives a brooch to Bucciarati to act as a tracker. On their way to the belfry, Trish finally shows nervousness at the idea of meeting her estranged father but Bucciarati does his best to reassure her. Suddenly, Trish disappears, with only her hand remaining in Bucciarati's palm. He thus realizes with horror that the Boss wanted to kill Trish personally. Bucciarati's past is revealed: once the ordinary son of a fisherman, Bucciarati's life took a turn for the worse when his father was gunned down by drug dealers when he saw an illegal trade happening. Bucciarati then had to kill them to protect his father and consequently had to join Passione for protection. Soon he discovered that Passione itself was the origin of the drugs. Utterly disgusted, Bucciarati decides to kill the Boss and gives chase. But, when Bucciarati hides in a columbarium to ambush the Boss, the latter somehow knows where he is. Bucciarati tries to attack, but his every move are foiled. As he sees a reflection of himself, the Boss and his King Crimson appear to explain their power to erase time and punch him in the guts.
Back to the boat, Giorno and his teammates wait for Bucciarati's return, but when events seem to have happened without anyone remembering performing them, Giorno rushes to the church to help Bucciarati. Meanwhile, the capo gets punched through the stomach by King Crimson but amazingly continues to fight. King Crimson erases time again, effortlessly avoiding Sticky Fingers' punch and lands a karate chop that splits Bucciarati's torso in two. However, the Boss is then sucked into the brooch Giorno has given life to. Bucciarati wins enough time to grasp the gist of King Crimson's power and tries to get rid of the turtle, but when he tries to crawl up the stairs with Trish, King Crimson blocks his path, having forecasted the attack and avoided it too. Fortunately, Bucciarati tricks King Crimson into letting him create a zipper to the ceiling and he escapes, meeting Giorno. Confusion ensues as Giorno realizes Bucciarati is physically dead, but he continues moving as if he was alive. With too many witnesses, the Boss decides to retreat. Out of the church, Bucciarati explains what has happened and gives his team the chance to join him in his fight against the Boss. The team is frightened, but all save Fugo manage to gather the resolve to follow their leader. Meanwhile, the operative Squalo receives the order to hunt the rebels.
Still in Venice, Team Bucciarati goes to a restaurant to rest and decide their next course of action. Sure that they can learn about the Boss's identity to ambush him and bypass his King Crimson, the team learns from Trish that the Boss had met her mother in Sardinia and thus the island is where they can learn about him. Narancia is suddenly attacked by a shark Stand named Clash, which rips off his tongue. Unfortunately, Clash can teleport between liquids and remains unseen by all but Narancia. Moreover, when Giorno saves him from suffocating and gives him a new tongue, Narancia begins to only tell lies, giving his teammates falsehoods. Indeed, a duo of Passione operatives, Tiziano and Squalo, are ambushing the group, using Tiziano's Talking Head to force Narancia to lie and bait them into watery places where Clash can ambush them. Narancia narrowly prevents his team from staying in the bathrooms too long, and they all leave but Giorno. Talking Head cuts Narancia's hand but when Giorno approaches, Narancia uses Aerosmith to shoot on a pipe and heat it with incendiary ammo and cauterize himself. Giorno understands that Narancia is under the effect of some power, but the damaged pipe leaks water, creating a puddle from which Clash emerges to tear at Giorno's throat.
Tiziano and Squalo's ambush on the heroes continue. Clash has managed to bite into Giorno's throat and has disabled him, dragging him along in the puddles while evading Aerosmith's bullets. Nonetheless, the duo are aware that Narancia can detect CO2 and thus track Giorno. Although Squalo baits Narancia into opening the way to the sewers, Giorno willingly lets himself get shot so that the tracer bullets can emit CO2 for him. Thus Narancia is able to chase Clash until it is cornered in the kitchen room. Despite the leaking gas preventing him from shooting Narancia slashes at Clash with Aerosmith's propellers. Tiziano intervenes and uses Talking Head to bait the team into the kitchen room and trick Mista into setting the gas on fire by firing his gun. An explosion ensues and Giorno uses the opportunity to tell him to find the User and gives him a brooch before Clash can take him. Narancia listens and gives chase to Tiziano and Squalo until he successfully finds them. Despite Tiziano's sacrifice, Narancia finally kills both of the enemies and finds Giorno barely alive. Team Bucciarati subsequently crosses the sea to an airport in order to fly to Sardinia.
Having managed to escape Venice, Team Bucciarati now heads to the Venezia-Tessera Airport and steals a passenger plane to fly to Sardinia. During the preparations, a Passione operative named Carne confronts them but Mista seemingly makes short work of him, only leaving him the time to briefly summon his Stand. Giorno touches him and confirms his death. Once the plane is up in the air, Trish and Bucciarati discuss their situation and Trish mentions Cala di Volpe, a small Sardinian bay in which her mother met the Boss, who was a local. Meanwhile, Giorno and Mista discover some of Carne's fingers, which got into the fridge of the plane without explanation. Giorno then discovers that his right arm is being absorbed by an enemy Stand. Notorious B.I.G, Carne's Stand, activates after death, can devour all energy and matter to grow, and is automatically attracted to the fastest object in the vicinity. Before Giorno can make it get out of the plane, he loses both arms and Mista and Narancia are heavily injured. The situation is extremely dire as half of the team is gravely injured with no healers available. Yet, as Bucciarati goes to the cockpit with the injured in the turtle to inform Abbacchio of what happened, Trish is left alone with Notorious B.I.G, which has managed to catch up to the plane. Trapped, Trish then sees that Giorno has left a brooch from which a brand new hand is growing.
Trish is trapped with Notorious B.I.G but must find a way to protect Giorno's growing hand and herself at the same time. Using the automatic reclining seats, Trish manages to go past the enemy Stand and approach the hand. However, Notorious B.I.G suddenly attacks her because of the droplets of drink slipping on her boot. Trish is nonetheless saved by her newly awakened Stand, Spice Girl, which can soften things. Using the softening, Spice Girl manages to bait Notorious B.I.G into attacking a clock without damaging it and seemingly destroys it. However, a tiny part of Notorious B.I.G reaches the engines, feeds on the energy in them and grows into a big size. Trish again saves the situation by softening the cockpit and then separate it from the crashing plane, acting as a makeshift parachute. Finally, the group manages to reach Sardinia and Giorno has regained his power. For his part, Notorious B.I.G will become in time a sea monster roaming the waters near Sardinia. Meanwhile, the Boss discovers the existence of a clue to his past in Sardinia. Sensing that Trish is alive, he decides to personally go to his native island.
Back in 1965, a baby boy was born from a woman who just spent two years in prison, surrounded by women. This unique baby was then raised in Sardinia by a priest, grew into a young man and eventually met Donatella Una. However, the priest discovered that his foster son had kept his mother prisoner and hidden under his room. The boy disappeared, but not before killing the priest and burning his home to erase all traces of him. Back to the present, a teen named Vinegar Doppio comes to Sardinia in order to reach the Costa Smeralda and watch it. Revealed to share his body with the Boss himself, Doppio manages to learn that Risotto is near but is nonetheless surprised by assassin. Risotto quickly sees that Doppio is a Stand user as he looked at the distant Aerosmith but hasn't guesses his true nature yet. A fight ensues, during which Risotto makes razors and nails appear in Doppio's body before disappearing. The Boss then communicates with Doppio and tells him to approach the assassin, granting a part of King Crimson's power to help him.
The fight between Doppio and Risotto Nero continues. Using Epitaph's power to predict the future, Doppio manages to hit close to the now invisible Risotto and extracts scissors safely from his throat. Doppio then guesses that Risotto is using magnetism to create the razors from his body's iron. Thus he discovers Risotto's location and severs the assassin's foot. However, Risotto recomposes himself, harasses Doppio with scalpels made from the iron in the rocks and takes so much iron from Doppio that the boy suffers from anemia. As Doppio is tricked into throwing his scalpels in the wrong direction, Risotto reveals himself and creates a razor to cut into Doppio's head. Having guessed that Doppio and the Boss are split personalities and seemingly having defeated his enemy, Risotto is nonetheless suddenly shot down by Aerosmith. The Boss then appears, having revealed that he purposefully told Doppio to throw the knives at Team Bucciarati on the beach, using his anemia to hide from Aerosmith's CO2 radar. Outplayed, Risotto collapses.
Having been attacked, Bucciarati and Narancia decide to scale the cliffs to check on the enemy while Abbacchio rewinds the past with Moody Blues. Meanwhile, the Boss orders Risotto to give him back his blood but the assassin attempts a last ditch attack, which fails because King Crimson erases time. Risotto thus dies. The Boss now has to escape from Bucciarati and Narancia, who notice that someone else was here and proceed to search the area. The two eventually find someone, but it is revealed that it is a boy that someone has drained the blood off. Meanwhile, Abbacchio approaches a group of kids and helps them retrieve a ball. Unfortunately, the Boss was hidden among them and kill Abbacchio. Abbacchio subsequently finds himself in the afterlife, where his former partner assures him that at the end, he upheld the morals his had. Team Bucciarati all grieve for Abbacchio but must leave the beach. However, Giorno sees that Abbacchio is holding a piece of rock, eventually discovering a negative of the Boss' face and fingerprints that Moody Blues has created. Abbacchio has fulfilled his mission.
After having acquired a mould of the Boss' face and fingerprints, Team Bucciarati research online if they correspond to anyone. Their efforts catch the attention of an unidentified man who calls them and convinces them that they are allies in the fight against the Boss, whose name is Diavolo. Showing them the Arrow and explaining the origins of Stands, this unknown man claims that the Arrows can unlock another power that could beat King Crimson. Interested, Bucciarati agrees to meet with the man in the Colosseum, in Rome. However, Diavolo learns that the team is up to something and thus calls forth his worst enforcers: Cioccolata and Secco, Cioccolata being a murderous surgeon and Secco his literal human pet. When the team arrive at a coastal village, they and the whole village begin to be affected by strange mold that grows when the victim goes down. It is Green Day, Cioccolata's Stand in action. Narancia jumps down into the team's boat, resulting in the near-death of almost everyone. Thankfully, Mista shoots the boat engine to make it explode and push Narancia up to the shores. This feat catches the interest of Cioccolata and Secco, who are filming the whole ordeal from a vantage point.
The fight against Cioccolata and Secco continues. Cioccolata now sics Secco on the team. Meanwhile, Giorno heals Narancia in the turtle, and Narancia has a conversation with Trish about Bucciarati's leadership. When Bucciarati and Mista ascend some stairs, the whole infrastructure begins to sink under the influence of Secco's Stand Oasis which can turn rock to sludge. While Mista and Bucciarati reach the parking lot, Secco continues to pursue and eventually wounds Mista. Bucciarati then jumps down the parking lot, surprising Secco and pummeling him to force him to retreat. Secco dives underground and then explains to Cioccolata that Bucciarati might be a walking corpse as he wasn't affected by the mold. Meanwhile, the group has already stolen a car and are driving to Rome. Giorno and Bucciarati use a private moment to discuss further Bucciarati's strange condition, and Bucciarati takes his status as a small blessing that allows him to fulfill his mission. They are interrupted when a mold-riddled corpse falls from them: Cioccolata and Secco have caught up in an helicopter, and the mold begins to massacre Rome's population. Secco dives to the ground but Giorno and Mista team up to stop Cioccolata's helicopter at the top of a tower. The team subsequently splits up to defeat both Stand Users.
The fight against Cioccolata and Secco continues. Bucciarati has decided to handle Secco, but he then discovers that Secco has him beat in strength and speed. Overwhelmed, Bucciarati must flee in the streets of Rome. For his part, Cioccolata is stuck in his helicopter and sees Giorno and Mista arrive for him. However, when Mista sends five of the Sex Pistols to kill Cioccolata, they notice that he's disappeared and get ambushed, resulting in Mista being taken out. Giorno thus goes to rescue the Pistols with no.5 but also gets ambushed because Cioccolata has managed to cut himself into piece and survive thanks to his medical knowledge. Green Day pummels Giorno out of the helicopter but Giorno ultimately makes no.5's bullet ricochet against the blades of the helicopter and shoot into Cioccolata's head, taking him out. Cioccolata attempts a last gambit by playing dead but is ultimately foiled, and Gold Experience violently pummels him into a garbage truck. Cioccolata is defeated. Secco then receives a voicemail from Cioccolata who encourages him and Secco comes out to fight Bucciarati. Unbeknownst to them, the mysterious ally is already observing them.
The battle against Secco continues. Bucciarati is forced to retreat but is caught up. The two then discover that Cioccolata has died by observing the crowd, but Secco surprisingly takes it well as he reveals that he only followed the surgeon for his wealth and treats. Secco nonetheless decides to go to the Coliseum to take what Bucciarati is after. Bucciarati is forced to stop him but another fistfight cements Secco's superiority over Sticky Fingers. Thus Bucciarati goes underground and zips him way through the soil to join the Coliseum, Secco swimming after him. Using his hearing, Secco relentlessly tracks Bucciarati and when Bucciarati decides to stand still, Secco then creates spikes of hardened rock that he makes rain down on the softened ground. Meanwhile, it is revealed that Jean Pierre Polnareff is the mysterious ally and that he must protect his Arrow's secret at all cost. Bucciarati is forced to move and is cornered but a car is sunk near him and he has Sticky Fingers punch the car's tire. The explosion bursts Secco's eardrums while Bucciarati is past caring about them. Resurfacing, Secco incidentally meets Doppio and takes him hostage but Bucciarati takes him out. Bucciarati then collapses as his body has reached its limits, and Doppio approaches to execute Bucciarati for good.
Instead of killing Bucciarati, Doppio decides to pass off as a harmless civilian to "help" carry Bucciarati to his destination. Diavolo enhances the ploy by deducing that Bucciarati is now a corpse who can only see spirits and emitting a bit of his own soul - Bucciarati is fooled into thinking Doppio is Trish. The two enter the Colosseum where they are confronted by Polnareff from the second floor. Polnareff eventually sees Doppio as a suspicious person, and both him and Diavolo remember Polnareff's attempt to investigate the Arrows in Italy only to be nearly killed by Diavolo in person. Polnareff forces Diavolo's hand, forcing the Boss to come out and confront him. Diavolo gets the better of Polnareff a second time, and Polnareff is forced to pierce Silver Chariot with the Arrow in front of Diavolo to protect it. Diavolo kills Polnareff and takes the Arrow. Looking down on the first floor, he sees Team Bucciarati coming to their leader and plans to kill them, but he then sees a new Stand in front of him.
Diavolo has taken the Arrow from Polnareff's corpse but is now seeing a new Stand in black walking in front of him. Soon, the entirety of Rome is put to sleep, the Stand takes the Arrow and a few hours pass. When the team wakes up, they realize that they have switched bodies. Giorno with Narancia and Mista with Trish, to Trish' dismay but the switch doesn't look like an attack as the group is otherwise fine and they discover that there Stand abilities have been empowered. The team then meets with Polnareff, whose soul was put in the turtle Coco Jumbo. Polnareff presents himself and explains that the Arrow unlocks greater power, narrating the incident allowing him to discover that Silver Chariot could gain the power to control souls. He's been forced to completely turn Silver Chariot into Chariot Requiem and the group must now defeat it to take the Arrow. He also warns that Diavolo may be working alongside a partner. The heroes spot Diavolo and Chariot Requiem and prepare to attack - however, Diavolo then summons Sticky Fingers to attack Chariot Requiem, meaning Bucciarati is in the Boss' body.
Bucciarati (who is in Diavolo's body) has seemingly taken out Chariot Requiem and severed his arm. However, the team soon discovers that when they try to approach the Arrow, their Stands automatically begin to attack them. Thus, Chariot Requiem is free to take back his arm and the Arrow and walk away. The team also realize that Diavolo must be now in Bucciarati's body and shoot him when he wakes up. Despite Requiem, all seems well and Narancia thinks about his future. However, time is erased again and the team realize that Narancia has been impaled on iron bars. It is too late for Narancia and the team can only mourn their lost companion. Giorno also regains his body. For his part, Polnareff deduces that Diavolo and Doppio were split personalities, and that Diavolo's soul may be lurking nearby. The group must depart to catch up to Chariot Requiem. In the streets, Bucciarati experiments with Requiem, and Polnareff tries to grab the Arrow since he's Standless. This spurs Chariot Requiem into action who now runs towards the turtle.
The struggle to defeat Chariot Requiem continues. After Polnareff touches the Arrow, Giorno and Mista must protect him from Chariot Requiem and Mista must replace his inexplicably broken revolver with a pistol. After a short struggle, Chariot stops running after Polnareff and goes away with the Arrow, only for it to be revealed that everyone is beginning to mutate into otherworldly creatures because of Chariot. Meanwhile, Diavolo has seen Chariot's weakness. Time presses but Giorno suddenly tells everyone to be still: he's checked Mista's revolver and proves that it was sabotaged; from that he deduces that Diavolo is hiding into one of them. When Giorno checks on Bruno, Diavolo manifest from the same body as his daughter and attacks Giorno, severing him arm. Diavolo then catches up to Chariot Requiem, using time erasure to evade all attacks. In truth, a hidden light orb can be attacked behind each soul's head and King Crimson punches his orb to disable Chariot and take the Arrow. The group tries to prevent Diavolo from taking the Arrow, culminating in it being send in the air but King Crimson punching through his host's stomach to send himself flying toward the Arrow and kill Trish once and for all
Diavolo almost grabs the Arrowhead, but Bucciarati intervenes. He has too seen through Chariot Requiem and definitely destroys the light orb behind him to obliterate Chariot, forcing Diavolo's soul away from the Arrow. Everyone returns to their original body and Giorno is able to heal Mista's body. Moreover, Giorno witnesses Bucciarati's soul ascending to heaven. Bucciarati explains how Giorno affected his life for the better and entrusts to him the end of their mission. Giorno thus takes the Arrow. Diavolo is bitter over his loss of the Arrow but is reassured as he forecasts Giorno being rejected. However, when Giorno stabs Gold Experience, it does turn into Gold Experience Requiem. GER performs a first attack but Diavolo foresees it and doesn't see any real change. Considering King Crimson to still be superior, Diavolo erases time. Foreseeing himself punching through Giorno, Diavolo is elated but his joy is crushed as he sees that time is rewinding back and that his time erasure is being undone. Diavolo finds himself at the same position he was just before the time erasure was activated and is powerless to prevent Gold Experience Requiem from starting to pummel him and King Crimson.